Figure 1. LncRNA SNHG22 was suppressed in OS and associated with tumor metastasis. (A) Volcano plot of differentially expressed genes in OS and lung metastases. (B) Heat map of the associated genes. Red represents upregulation. Green represents downregulation. (C) The qRT-PCR confirmed the decreased expression of SNHG22 in OS cell lines. Results are shown as means±SD. n=5; **P<0.01 by t-test. (D) qRT-PCR analysis of SNHG22 expression in the nucleus and cytoplasm of U2OS or HOS cells. Results are shown as means±SD. n=5; ***P<0.001 by t-test.