Research Paper

Figure 6. Silencing lncRNA CASC15 increases the radiosensitivity of ESCC in vivo. (A) RT-qPCR to detect the silencing efficiency of lncRNA CASC15 and the expression of miR-140-5p and MMP7 in tumors. (B) The volume of tumors following X-ray radiation and lncRNA CASC15 silencing. (C) The weight of tumors following X-ray radiation and lncRNA CASC15 silencing. (D) The levels of MMP7, Ki-67 and cleaved Caspase 3 in tumors in response to X-ray radiation and lncRNA CASC15 silencing determined by Western blot analysis. (E) The levels of MMP7, Ki-67 and cleaved Caspase 3 in tumors in response to X-ray radiation and lncRNA CASC15 silencing detected by IHC analysis (× 200). *p < 0.05 vs. the 4 Gy + sh-NC group. n = 12. The measurement data were presented as the mean ± standard deviation. Data in panel (A, C–E) were analyzed by unpaired t test and panel B by repeated-measures analysis of variance, followed by Tukey’s post hoc test; the experiment was repeated 3 times. 4 Gy + sh-CASC15, 4 Gy irradiated mice with EC109 cells infected with lentivirus containing shRNA targeting CASC15, 4 Gy + sh-NC, 4 Gy irradiated mice with EC109 cells infected with lentivirus containing negative control shRNA.