Research Paper Volume 12, Issue 18 pp 18635—18648

Visfatin increases ICAM-1 expression and monocyte adhesion in human osteoarthritis synovial fibroblasts by reducing miR-320a expression

Figure 3. Visfatin stimulates ICAM-1 expression and monocyte adhesion in OASFs. (AC) OASFs were incubated with visfatin (1–30 ng/mL) for 24 h, and ICAM-1 expression was examined by RT-qPCR, Western blot and ELISA analysis. (D) OASFs were incubated with visfatin (1–30 ng/mL) for 24 h. THP-1 cells loaded with BCECF-AM were added to OASFs for 6 h, then THP-1 cell adherence was measured by fluorescence microscopy. * p<0.05 compared with the control group.