Research Paper Volume 12, Issue 18 pp 18635—18648

Visfatin increases ICAM-1 expression and monocyte adhesion in human osteoarthritis synovial fibroblasts by reducing miR-320a expression

Figure 8. Knockdown visfatin reduces ICAM-1 expression and monocyte adhesion in OASFs. (A, B) OASFs were transfected with visfatin shRNA. Visfatin and ICAM-1 expression was examined by Western blot and ELISA. (C) OASFs were treated with the same conditions as those described in (A). THP-1 cells loaded with BCECF-AM were added to OASFs for 6 h, then THP-1 cell adherence was measured by fluorescence microscopy. (D) The schematic diagram summarizes the mechanism whereby visfatin promotes ICAM-1 expression and monocyte adhesion in OASFs. Visfatin promotes ICAM-1 expression and enhances monocyte adhesion to OASFs by downregulating miR-320a through the AMPK and p38 signaling pathways. * p<0.05 compared with the control group.