Research Paper Volume 13, Issue 9 pp 13333—13348

Sevoflurane protects against ischemia-reperfusion injury in mice after total knee arthroplasty via facilitating RASD1-mediated protein kinase A pathway activation

I/R injury is inhibited by Sevoflurane treatment in mice following TKA. (A) Levels of IFN-γ, IL-6 and TNF-α in serum of mice measured by ELISA. (B) HE staining of knee joint tissues of mice (× 200). (C) Cell apoptosis in knee joint tissues of mice measured by TUNEL assay (× 200). (D) Protein expression patterns of proliferation- and apoptosis-markers examined by Western blot analysis. * p p

Figure 1. I/R injury is inhibited by Sevoflurane treatment in mice following TKA. (A) Levels of IFN-γ, IL-6 and TNF-α in serum of mice measured by ELISA. (B) HE staining of knee joint tissues of mice (× 200). (C) Cell apoptosis in knee joint tissues of mice measured by TUNEL assay (× 200). (D) Protein expression patterns of proliferation- and apoptosis-markers examined by Western blot analysis. * p < 0.05 vs. sham-operated mice; # p < 0.05 vs. I/R mice. The above data were all measurement data and expressed as mean ± standard deviation. Data among multiple groups were compared by one-way ANOVA, followed by a Tukey’s multiple comparisons posttest. The experiment was repeated 3 times independently. Normal mice or sham-operated mice: n = 15; I/R mice or I/R mice treated with Sevoflurane: n = 10.