Figure 2. RASD1 is expressed at a high level in I/R injury and Sevoflurane treatment suppresses its expression. (A) The expression pattern of RASD1 following model establishment and Sevoflurane treatment detected using RT-qPCR. (B) Immunohistochemistry analysis of RASD1 expression patterns following model establishment and Sevoflurane treatment (× 200). Normal mice or sham-operated mice: n = 10; I/R mice or I/R mice treated with Sevoflurane: n = 10. (C) RASD1 expression patterns in the skeletal muscle cells after HRO and Sevoflurane treatment detected using RT-qPCR. (D) Western blot analysis of the RASD1 expression patterns in skeletal muscle cells after HRO and Sevoflurane treatment. * p < 0.05 vs. sham-operated mice or control cells; # p < 0.05 vs. I/R mice or HRO cells. The above data were all measurement data and expressed as mean ± standard deviation. Data among multiple groups were compared by one-way ANOVA, followed by Tukey’s multiple comparisons posttest. The cell experiment was repeated 3 times independently.