Research Paper Volume 13, Issue 4 pp 4986—4998

Loss of FBP1 promotes proliferation, migration, and invasion by regulating fatty acid metabolism in esophageal squamous cell carcinoma

MiR-18b-5p inhibitor regulated loss of FBP1 induced fatty acid metabolism in Eca109 cells. (A) The Eca109 cell proliferation was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. (B) The Eca109 cell proliferation was determined following treatment with shFBP1 and/or miR-18b-5p mimic. (C) The Eca109 cell invasion was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. (D) The Eca109 cell migration was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. (E) The content of phospholipids was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. (F) The content of triglycerides was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. (G) The neutral lipids content was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor by staining with BODIPY 493/503 dye (green) and DAPI (blue) in Eca109 cells, scale bar= 20μm. (H, I) The protein expression and quantification of FASN, ACC1 and SREBP1C was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. *p

Figure 5. MiR-18b-5p inhibitor regulated loss of FBP1 induced fatty acid metabolism in Eca109 cells. (A) The Eca109 cell proliferation was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. (B) The Eca109 cell proliferation was determined following treatment with shFBP1 and/or miR-18b-5p mimic. (C) The Eca109 cell invasion was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. (D) The Eca109 cell migration was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. (E) The content of phospholipids was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. (F) The content of triglycerides was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. (G) The neutral lipids content was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor by staining with BODIPY 493/503 dye (green) and DAPI (blue) in Eca109 cells, scale bar= 20μm. (H, I) The protein expression and quantification of FASN, ACC1 and SREBP1C was determined following treatment with shFBP1 and/or miR-18b-5p inhibitor. *p< 0.05 VS control, **p< 0.01 VS control, # p< 0.05 VS shFBP1, ## p< 0.01 VS shFBP1.