Research Paper Volume 12, Issue 18 pp 18693—18715

Resemblance and differences in dietary restriction nephroprotective mechanisms in young and old rats

Figure 3. Assessment of autophagic activity in young and old rats. (A) LC3 II/LC3 I ratio in kidney tissue of young rats in response to DR; (B) LC3 II/LC3 I ratio in kidney tissue of young rats in response to I/R; (C) The comparison of LC3 II/LC3 I ratio in kidney tissue of young rats after I/R with or without DR diet; (D) LC3 II/LC3 I ratio in kidney tissue of old rats in response to DR; (E) LC3 II/LC3 I ratio in kidney tissue of old rats in response to I/R; (F) The comparison of LC3 II/LC3 I ratio in kidney tissue of old rats after I/R with or without DR diet; (G) Comparison of LC3 II/LC3 I ratio in kidney tissue of young and old rats; (H, I) Levels of non-glycosylated (40 kDa) and glycosylated (120 kDa) forms of LAMP1 in kidney tissue of young rats; (J, K) Levels of non-glycosylated (40 kDa) and glycosylated (120 kDa) forms of LAMP1 in kidney tissue of old rats; (L) Lysosomes staining with LysoTracker Green probe revealed by confocal microscopy of vital kidney slices. Scale bar, 100 μm; (M) Quantification of LysoTracker Green fluorescence intensity. *p < 0.05 compared to AL-group. For young AL rats n=5, for young DR rats n=6, for old AL rats n=6, for old DR rats n=5. AL, ad libitum, DR, dietary restriction, I/R, ischemia/reperfusion.