Research Paper Volume 12, Issue 18 pp 18693—18715

Resemblance and differences in dietary restriction nephroprotective mechanisms in young and old rats

Figure 6. ROS production and oxidative stress in the kidney. (A) Confocal microscopy of DCF-loaded vital kidney slices. Scale bar, 100 μm; (B) Quantification of DCF fluorescence intensity on confocal images; (C) OxyBlot staining for carbonylated proteins in kidney tissue of young DR rats; (D) OxyBlot staining for carbonylated proteins in kidney tissue of old rats after DR; (E) Comparison of carbonylated protein levels in kidney tissue of young and old rats; (F) Confocal microscopy of vital kidney slices stained with Lipid Peroxidation Kit. Scale bar, 100 μm; (G) Estimation of lipid peroxidation measured by a green/red ratio of fluorescence intensity. *p < 0.05 compared to young AL-group, *p < 0.05 compared to old AL-group. For young AL rats n=5, for young DR rats n=6, for old AL rats n=6, for old DR rats n=5. AL, ad libitum, DR, dietary restriction.