Research Paper Volume 12, Issue 24 pp 25020—25034

TRIB3 facilitates glioblastoma progression via restraining autophagy

Figure 6. TRIB3-mediated suppression of autophagy promotes the proliferation and migration of GBM cells. (A, B) Migration ability (A) and invasion ability (B) were determined after the downregulation of TRIB3 coupled with downregulation of ATG5 or ATG7 in GBM cells using a migration assay and a Transwell assay. (C) Relative migration distance was determined in GBM cells using a migration assay. (D) Migration ability and invasion ability were determined after downregulation of TRIB3 and treatment with the autophagy inhibitor CQ or vehicle (DMSO) in GBM cells using a migration assay and a Transwell assay. E, Relative migration distance was determined in GBM cells using a migration assay. *p<0.05, **p< 0.01, #p< 0.05 vs. TRIB3 KD + sh-NC.