LGALS1 enhances GLI1 expression in vivo. (A) GLI1 and GAL-1 IHC scores were positively correlated in subcutaneous GC tissues from all groups (P < 0.05; ×200 magnification). (B) IHC scores indicating that GLI1 expression was elevated in the OE-LGALS1 group (P < 0.01). (C) IHC scores indicating that GLI1 expression was abrogated in the shLGALS1#3 group (P < 0.01). LGALS1 and GLI1 mRNA expression levels in (D) OE LGALS1-transduced and untransduced cells, and (E) shLGALS1#3-transduced and untransduced cells.