Research Paper Volume 12, Issue 18 pp 18716—18740
Identification of robust genetic signatures associated with lipopolysaccharide-induced acute lung injury onset and astaxanthin therapeutic effects by integrative analysis of RNA sequencing data and GEO datasets
Figure 5. Changes in the expression of 11 core genes (A-K: Saa3, Ly6i, Saa1, Irf7, Cxcl5, Ccl7, Timp1, Isg15, Gbp11, Tgtp1, and Cxcl13) in the lungs of an established mouse model. Quantitative real-time polymerase chain reaction (RT-qPCR) was conducted to quantify the relative expression of the 11 core genes in the control group (n = 4), LPS-induced ALI group (n = 4), and AST treatment group (n = 4). GAPDH was used as an internal control gene. The experiments were performed at least in triplicate. The error bars represent the SEM. Statistical analysis of significant differences between groups was achieved with one-way ANOVA using Prism 7 software. ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 were considered statistically significant.