Research Paper Volume 12, Issue 22 pp 23082—23095

Deubiquitinase USP7 regulates Drosophila aging through ubiquitination and autophagy

Figure 8. Effect of DMC addition on protein degradation in dusp7-knockdown Drosophila. (A) Expression level of ubiquitin protein (*** p<0.001). (B) Expression level of autophagy-associated gene (* p<0.05; ***p<0.001). (C) Expression level of autophagy-associated protein (* p<0.05; *** p<0.001). (D) Number of autolysosomes (** p<0.01; *** p<0.001; white arrow points to autophagosome). (>: hybridization; + means adding corresponding ingredients; - means no adding corresponding ingredients).