Research Paper Volume 12, Issue 24 pp 25120—25137

Apelin protects against ischemia-reperfusion injury in diabetic myocardium via inhibiting apoptosis and oxidative stress through PI3K and p38-MAPK signaling pathways

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Figure 2.

Observation for the apoptosis and associated biomarkers. (A) Apoptosis in myocardium was determined by TUNEL analysis. Scale bar=50 μm. (B) mRNA levels of Bcl-2, Bax, and cleaved caspase-3 detecting by RT-qPCR. *P<0.05, **P<0.01, ***P<0.001 represents the values compared to the ND-Sham group; #P<0.05, ##P<0.01 represents the values compared to D-Sham. (C) Protein levels of Bcl-2, Bax, and cleaved caspase-3 detecting by western blot assay. **P<0.01, ***P<0.001 represents the values compared to the ND-Sham group; ###P<0.001 represents the values compared to D-Sham. ND-Sham: Non-diabetes sham group; ND-IRI: Non-diabetes ischemia reperfusion injury; D-IRI: Diabetes ischemia reperfusion injury; D-Sham: Diabetes sham group.