Research Paper Volume 12, Issue 24 pp 25120—25137

Apelin protects against ischemia-reperfusion injury in diabetic myocardium via inhibiting apoptosis and oxidative stress through PI3K and p38-MAPK signaling pathways

Figure 6. Observation for the apoptosis reduced by the injection of recombined lentiviral vector expressing Apelin. (A, B) Apoptosis in myocardium was determined by TUNEL analysis. Scale bar=50 μm. (C, D) Protein levels and analysis of Bcl-2, Bax, and cleaved caspase-3 detecting by western blot assay. (E) mRNA levels of Bcl-2, Bax, and cleaved caspase-3 detecting by RT-qPCR. * P<0.05, *** P<0.001 represent the values compared to the D-Sham group. ###P<0.001 represents the values compared to Ad-GFP group. D-Sham: Diabetes sham group; D-IRI: Diabetes ischemia reperfusion injury; Ad-GFP: D-IRI rats injected with Ad-recombined lentiviral vector expressing GFP. Ad-Apelin: D-IRI rats injected with Ad-recombined lentiviral rector expressing Apelin.