Figure 6. Observation for the apoptosis reduced by the injection of recombined lentiviral vector expressing Apelin. (A, B) Apoptosis in myocardium was determined by TUNEL analysis. Scale bar=50 μm. (C, D) Protein levels and analysis of Bcl-2, Bax, and cleaved caspase-3 detecting by western blot assay. (E) mRNA levels of Bcl-2, Bax, and cleaved caspase-3 detecting by RT-qPCR. * P<0.05, *** P<0.001 represent the values compared to the D-Sham group. ###P<0.001 represents the values compared to Ad-GFP group. D-Sham: Diabetes sham group; D-IRI: Diabetes ischemia reperfusion injury; Ad-GFP: D-IRI rats injected with Ad-recombined lentiviral vector expressing GFP. Ad-Apelin: D-IRI rats injected with Ad-recombined lentiviral rector expressing Apelin.