Research Paper Volume 12, Issue 24 pp 25189—25206

Histone methyltransferase SETD2 inhibits tumor growth via suppressing CXCL1-mediated activation of cell cycle in lung adenocarcinoma

Figure 7. SETD2-CXCL1 axis inhibits the cell proliferation ability and cell cycle in LUAD cells in vitro. (A) Cell proliferation and colony formation assay (B) evaluated the effect of exogenous CXCL1 treatment in SETD2 overexpressed LUAD cells, compared with vector control LUADs cells. (C) Cell proliferation and colony formation assay (D) evaluated the effect of endogenous CXCL1 neutralization by Anti-CXCL1 antibody in SETD2 deficiency LUAD cells, compared with vector control LUADs cells. (E) Cell cycle analysis of the effect of exogenous CXCL1 treatment in SETD2 overexpressed LUAD cells, compared with vector control LUADs cells. (F) Cell cycle analysis of the effect of endogenous CXCL1 neutralization by Anti-CXCL1 antibody in SETD2 deficiency LUAD cells, compared with vector control LUADs cells. *P<0.05. One-way ANOVA with Dunn’s multiple-comparisons test.