Research Paper Volume 12, Issue 24 pp 25229—25255

Exposure to ozone impacted Th1/Th2 imbalance of CD4+ T cells and apoptosis of ASMCs underlying asthmatic progression by activating lncRNA PVT1-miR-15a-5p/miR-29c-3p signaling

Figure 7. PI3K/AKT/mTOR signaling mediated the impact of PVT1/miR-29c-3p axis on ASMC activity. (A) Expressions of p-PI3K, PI3K, p-AKT, AKT, p-mTOR and mTOR were determined within ASMCs that were treated by none, pcDNA3.1-PVT1, si-PVT1, miR-29c-3p mimic and miR-29c-3p inhibitor. *: P<0.05 when compared with NC group. (B) Expressions of PVT1 and miR-29c-3p were assessed in ASMCs managed by IGF-1 and LY294002. *: P<0.05 when compared with NC group. (CE) Viability (C), proliferation (D) and apoptosis (E) of ASMC were appraised among miR-NC, miR-29c-3p mimic and miR-29c-3p mimic+IGF-1 groups. *: P<0.05 when compared with miR-NC group, #: P<0.05 when compared with miR-29c-3p mimic group. (F) Expressions of apoptins were measured within ASMCs of miR-NC, miR-29c-3p mimic and miR-29c-3p mimic+IGF-1 groups. *: P<0.05 when compared with miR-NC group, #: P<0.05 when compared with miR-29c-3p mimic group.