Research Paper Volume 12, Issue 24 pp 25452—25468

Sevoflurane postconditioning reduces myocardial ischemia reperfusion injury-induced necroptosis by up-regulation of OGT-mediated O-GlcNAcylated RIPK3

Figure 4. SPC increased O-GlcNAcylated RIPK3 and inhibited the combination of RIPK3 and MLKL. (A) RIPK3 and MLKL were detected in O-GlcNAc immunoprecipitates. Representative protein images were performed. (B) Quantitative analysis of O-GlcNAcylated RIPK3 and MLKL. n = 3 /group. (C) O-GlcNAc and MLKL in RIPK3 immunoprecipitates. Representative protein images were shown. (D) Quantitative analysis of O-GlcNAcylated RIPK3 and the complex between RIPK3 with MLKL. n = 3 /group.