Research Paper Volume 12, Issue 24 pp 25452—25468

Sevoflurane postconditioning reduces myocardial ischemia reperfusion injury-induced necroptosis by up-regulation of OGT-mediated O-GlcNAcylated RIPK3

Figure 6. OSMI-1 abolished SPC induced activation in OGT mediated O-GlcNAcylation and reduction in p-RIPK3, p-MLKL. (A, B) Expressions of O-GlcNAc, OGT and OGA in all groups were analyzed by Western Blot. Representative protein images and quantitative analysis were shown. n=3/group. (C, D) Representative protein images and quantitative analysis of p-RIPK1, p-RIPK3, and p-MLKL in all groups were presented. n=3/group. OSMI-1, the OGT inhibitor; DMSO, the OSMI-1 solvent. The columns and errors bars represent means ± SD. * P < 0.05 vs. SHAM group; # P < 0.05 vs. IR group; & P < 0.05 vs. SPC group.