Figure 8. Schematic illustration of the experimental protocol in vivo and in vitro. (A) All the groups underwent the same surgical operation. (1) SHAM: rats were subjected to open chest surgery only; (2) SEVO: rats received 1.0 MAC sevoflurane for 15 min without occlusion; (3) I/R: rats were subjected to 30 min LAD occlusion, followed by 2 h of reperfusion; (4) SPC: rats were subjected to I/R and receiving 1.0MAC sevoflurane for 15 min at onset of reperfusion. (B) Except the SHAM group, all hearts was subjected to 30 min of global ischemia, followed by 2 h of reperfusion. SPC group received 1.0 MAC sevoflurane for 15 min at onset of reperfusion. In the SPC+DMSO group or SPC+OSMI-1 group, in addition to SPC administration, 50 μM DMSO or OSMI-1 was given and present throughout the experiment.