Research Paper Volume 13, Issue 1 pp 340—350

LINC00052 ameliorates acute kidney injury by sponging miR-532-3p and activating the Wnt signaling pathway

Analysis of LINC00052 and miR-532-3p expression in AKI rat models triggered by I/R surgery. (A) Renal histology micrographs of renal tissues from I/R-induced AKI rat models. Scale bars = 20 μm. (B) TUNEL assays measuring apoptosis in renal tissues from I/R-induced AKI rat models. Serum levels of SCr (C) and BUN (D) in renal tissues from I/R-induced AKI rats 24 h after surgery. (E) The expression of IL-1β, IL-6 and TNF-α mRNAs in renal tissues from I/R-induced AKI rats 24 h after surgery was assessed by qRT-PCR. Analysis of LINC00052 (F) and miR-532-3p (G) expression in renal tissues from I/R-induced AKI rats using qRT-PCR. Three independent experiments were performed (n = 8 in each group). Error bars represent the mean ± SD of triplicate experiments (at least). *p

Figure 2. Analysis of LINC00052 and miR-532-3p expression in AKI rat models triggered by I/R surgery. (A) Renal histology micrographs of renal tissues from I/R-induced AKI rat models. Scale bars = 20 μm. (B) TUNEL assays measuring apoptosis in renal tissues from I/R-induced AKI rat models. Serum levels of SCr (C) and BUN (D) in renal tissues from I/R-induced AKI rats 24 h after surgery. (E) The expression of IL-1β, IL-6 and TNF-α mRNAs in renal tissues from I/R-induced AKI rats 24 h after surgery was assessed by qRT-PCR. Analysis of LINC00052 (F) and miR-532-3p (G) expression in renal tissues from I/R-induced AKI rats using qRT-PCR. Three independent experiments were performed (n = 8 in each group). Error bars represent the mean ± SD of triplicate experiments (at least). *p < 0.05.