Research Paper Volume 13, Issue 1 pp 340—350

LINC00052 ameliorates acute kidney injury by sponging miR-532-3p and activating the Wnt signaling pathway

Figure 4. The impacts of LINC00520 and miR-532-3p on NRK-52E cells experiencing hypoxia. (A) LINC00052 expression in NRK-52E cells infected with LV-LINC00052 or LV-LINC00052 and LV-miR-532-3p under hypoxic conditions. (B) miR-532-3p expression in NRK-52E cells infected with LV-LINC00052 or LV-LINC00052 and LV-miR-532-3p. (C and D) NRK-52E cell proliferation was detected using an EdU assay. Cells were infected with a combination of LV-LINC00052 and LV-miR-532-3p or LV-LINC00052 and LV-miR-532-3p. (E and F) NRK-52E cell apoptosis was measured using flow cytometry. Three independent experiments were performed. Error bars represent the mean ± SD of triplicate experiments (at least). *p < 0.05.