Figure 4. Repression of autophagy inhibited the protective effect of Sesn2 in EPCs. EPCs were divided into treatment groups representing the control, CQ, Lenti-Sesn2, and Lenti-Sesn2 + CQ cohorts. (A) The levels of expression of proteins of Keap1, Nrf2, Cleaved-caspase3, Cleaved-caspase9, P62, LC-3, and GAPDH in EPCs were detected by use of Western blotting. (B) The levels of expression of proteins of Nrf2 in EPCs were determined by using Immunofluorescence. Scale bars = 2 μm. (C) Measures of the migration of EPCs were determined by using Transwell assays. All experiments were performed in triplicate. Scale bars = 5 μm. *p < 0.05, **p < 0.01, ***p < 0.001, versus the control. Data are represented as mean +/- SEM.