Figure 1. Differentially expressed MiRNAs of human spermatogonia between OA and NOA patients and transfection efficiency of ZsGreen1-labeled lentivirus in human SSC line. (A–D) Real-time qPCR revealed the different expression levels of miRNA-122-5p (miR-122-5p) (A), miR-373-3p (B), miR-100-5p (C), and miR-145-5p (D) in human spermatogonia between OA and NOA patients. * indicates statistically significant differences (p< 0.05) in human spermatogonia between human OA and NOA patients. (E) Fluorescence microscope and phase-contrast microscope indicated the transfection efficiency of ZsGreen1-labeled miRNA-122-5p lentivirus compared to the control lentivirus of human SSC line. Scale bars =40 μm. (F) Real-time qPCR showed the relative levels of miRNA-122-5p in human SSCs treated with miRNA-122-5p vector and control lentivirus. * indicated statistically significant differences (p< 0.05) in human SSC line between miRNA-122-5p vector and the control lentivirus.