Research Paper Volume 12, Issue 24 pp 25528—25546

miRNA-122-5p stimulates the proliferation and DNA synthesis and inhibits the early apoptosis of human spermatogonial stem cells by targeting CBL and competing with lncRNA CASC7

Figure 1. Differentially expressed MiRNAs of human spermatogonia between OA and NOA patients and transfection efficiency of ZsGreen1-labeled lentivirus in human SSC line. (A–D) Real-time qPCR revealed the different expression levels of miRNA-122-5p (miR-122-5p) (A), miR-373-3p (B), miR-100-5p (C), and miR-145-5p (D) in human spermatogonia between OA and NOA patients. * indicates statistically significant differences (p< 0.05) in human spermatogonia between human OA and NOA patients. (E) Fluorescence microscope and phase-contrast microscope indicated the transfection efficiency of ZsGreen1-labeled miRNA-122-5p lentivirus compared to the control lentivirus of human SSC line. Scale bars =40 μm. (F) Real-time qPCR showed the relative levels of miRNA-122-5p in human SSCs treated with miRNA-122-5p vector and control lentivirus. * indicated statistically significant differences (p< 0.05) in human SSC line between miRNA-122-5p vector and the control lentivirus.