Research Paper Volume 12, Issue 24 pp 25528—25546

miRNA-122-5p stimulates the proliferation and DNA synthesis and inhibits the early apoptosis of human spermatogonial stem cells by targeting CBL and competing with lncRNA CASC7

Figure 5. The effect of MiRNA-122-5p inhibitor and CBL silencing on DNA synthesis and proliferation of human SSC line. (A, B) EDU incorporation assay showed the percentages of EDU+ cells in human SSC line treated with miRNA inhibitor control, miRNA-122-5p inhibitor, as well as miRNA-122-5p inhibitor and CBL siRNA1. Scale bars = 50 μm. (C) CCK-8 assay showed the proliferation of human SSC line treated with miRNA inhibitor control, miRNA-122-5p inhibitor, and miRNA-122-5p inhibitor and CBL siRNA1 for 5 days. * denoted statistically significant differences in human SSC line (p< 0.05) between miRNA-122-5p inhibitor and the inhibitor control as well as the miRNA-122-5p inhibitor and miRNA-122-5p inhibitor plus CBL siRNA1.