Research Paper Volume 13, Issue 3 pp 3342—3352

The DNA methylation-regulated MCTP1 activates the drug-resistance of esophageal cancer cells

Effects of a forced reversal of the MCTP1 levels on the drug resistance of Kyse510 cells. (A) MCTP1 overexpression lentivirus vector map. (B) Representative areas of light and fluorescent of Kyse510 cells infected with MCTP1 overexpression lentivirus vector and the negative control (NC). MCTP1 mRNA (C) and protein (D) level determined by qRT-PCR and western blot analysis of Kyse510 cells infected with MCTP1 overexpression lentivirus vector and the negative control (NC). (E) The cell death triggered by an IC50 dose of four drugs in Kyse510 cells infected with MCTP1 overexpression lentivirus vector versus the negative control (NC) assayed 72hr after treatment with the IC50 dose of drugs.

Figure 4. Effects of a forced reversal of the MCTP1 levels on the drug resistance of Kyse510 cells. (A) MCTP1 overexpression lentivirus vector map. (B) Representative areas of light and fluorescent of Kyse510 cells infected with MCTP1 overexpression lentivirus vector and the negative control (NC). MCTP1 mRNA (C) and protein (D) level determined by qRT-PCR and western blot analysis of Kyse510 cells infected with MCTP1 overexpression lentivirus vector and the negative control (NC). (E) The cell death triggered by an IC50 dose of four drugs in Kyse510 cells infected with MCTP1 overexpression lentivirus vector versus the negative control (NC) assayed 72hr after treatment with the IC50 dose of drugs.