Research Paper Volume 12, Issue 24 pp 25658—25672

Up-regulation of circRNA-0003528 promotes mycobacterium tuberculosis associated macrophage polarization via down-regulating miR-224-5p, miR-324-5p and miR-488-5p and up-regulating CTLA4

Figure 4. Establishment of a molecular signaling pathway (*P value < 0.05 vs. NC group). (A) The expression of miR-224-5p in THP-1 cells transfected with hsa_circ_0003528 and the empty control; (B) The expression of miR-324-5p in THP-1 cells transfected with hsa_circ_0003528 and the empty control; (C) The expression of miR-488-5p in THP-1 cells transfected with hsa_circ_0003528 and the empty control; (D) The expression of CTLA4 mRNA in THP-1 cells transfected with hsa_circ_0003528 and the empty control; (E) The expression of CTLA4 protein in THP-1 cells transfected with hsa_circ_0003528 and the empty control; (F) The expression of hsa_circ_0003528 in U937 cells transfected with hsa_circ_0003528 siRNA and the negative control; (G) The expression of miR-224-5p in U937 cells transfected with hsa_circ_0003528 siRNA and the negative control; (H) The expression of miR-324-5p in U937 cells transfected with hsa_circ_0003528 siRNA and the negative control; (I) The expression of miR-488-5p in U937 cells transfected with hsa_circ_0003528 siRNA and the negative control; (J) The expression of CTLA4 mRNA in U937 cells transfected with hsa_circ_0003528 siRNA and the negative control; (K) The expression of CTLA4 protein in U937 cells transfected with hsa_circ_0003528 siRNA and the negative control.