Research Paper Volume 12, Issue 24 pp 25700—25717

Inhibition of PAR-1 delays aging via activating AMPK in C. elegans

Figure 2. RNAi knockdown of par-1 significantly improves healthspan. (A) Survival curves of wild-type animals treated with either the control or par-1 RNAi at 35°C (p < 0.0001, log-rank test). (B) Survival curves of wild-type animals treated with either the control or par-1 RNAi upon UV (2,000 J/m2) exposure (p = 0.0003, log-rank test). (C, D) Age-associated paralysis induced by body wall muscle expression of either Q35 (C) or Aβ (D) in animals treated with the control or par-1 RNAi (p < 0.0001, log-rank tests). (E) Body bending rates of day 2, 4, 6, 8, and 10 adults treated with either the control or par-1 RNAi (****, p < 0.0001, **, p = 0.0029, two-way ANOVA with Sidak's multiple comparison tests). Detailed quantitative data and statistical analyses are included in Supplementary Tables 25.