Research Paper Volume 12, Issue 24 pp 25700—25717

Inhibition of PAR-1 delays aging via activating AMPK in C. elegans

Figure 5. Inhibition of par-1 activates AMPK and decreases lipid levels in the metabolic tissue. (A, B) Immunoblots (A) and quantification (B) of phospho-AAK-2 (AMPKα) and tubulin using proteins extracted from dissected intestinal tissues of day 2 and day 6 adult animals treated with the control or par-1 RNAi. Ratio of the phospho-AAK-2 band intensity to that of tubulin was normalized to the control RNAi treated samples. Data are represented as mean ± SD based on three independent biological replicates. (C, D) Representative Oil Red O staining images (C) and quantification (D) of the staining signal in day 2 and day 6 adult animals treated with the control or par-1 RNAi. Data are represented as mean ± SD. ns, not significant, ****, p < 0.0001 (n = 40, two-way ANOVA with Sidak's multiple comparisons tests). Scale bar, 50 μm.