Research Paper Volume 12, Issue 24 pp 24604—24622

Control of lifespan and survival by Drosophila NF-κB signaling through neuroendocrine cells and neuroblasts

Figure 2. Activation of Toll pathway signaling in IPCs or neuroblasts shortens lifespan. (AD) Survival, median lifespan, and 10% max lifespan (A) and bodyweight (B) of dilp2-Gal4/+;UAS-CactusRNAi/+ males (blue), dilp5-Gal4/+;UAS-CactusRNAi/+ males (brown), wor-Gal4/+;UAS-CactusRNAi/+ males (orange), and +/+;UAS-CactusRNAi/+ (black) control males. Corresponding lifespan analysis (C) and bodyweight (D) for females. (EH) Survival, median lifespan, and 10% max lifespan (E) and bodyweight (F) of dilp2-Gal4/+;UAS-Dif/+ males (blue), dilp5-Gal4/+;UAS-Dif /+ males (brown), wor-Gal4/+;UAS-Dif/+ males (orange), and +/+;UAS-Dif/+ (black) control males. Corresponding lifespan analysis (G) and bodyweight (H) for females. (IL) Survival, median lifespan, and 10% max lifespan (I) and bodyweight (J) of dilp2-Gal4/UAS-Relish males (blue), wor-Gal4/UAS-Relish males (orange), and UAS-Relish/+ (black) control males. Corresponding lifespan analysis (K) and bodyweight (L) for females. Data information: statistics for curve comparisons are shown in the figure. Error bars represent mean ± SEM. *** p<.001, **** p<.0001, n.s. not significant (log-rank test). n = at least 100 flies for each genotype in lifespan experiments. n = 4 vials of 15 flies for each genotype for bodyweight measurements.