Research Paper Volume 12, Issue 24 pp 24604—24622

Control of lifespan and survival by Drosophila NF-κB signaling through neuroendocrine cells and neuroblasts

Figure 4. Inhibition of Toll pathway signaling in IPCs or neuroblasts increases lifespan. (AD) Survival, median lifespan, and 10% max lifespan (A) and bodyweight (B) of dilp2-Gal4/UAS-Cactus males (blue), dilp5-Gal4/UAS-Cactus males (brown), wor-Gal4/UAS-Cactus males (orange), dilp5-Gal4,wor-Gal4/UAS-Cactus males (magenta), and UAS-Cactus/+ (black) control males. Corresponding lifespan analysis (C) and bodyweight (D) for females. (EH) Survival, median lifespan, and 10% max lifespan (E) and bodyweight (F) of dilp2-Gal4/+;UAS-DifRNAi/+ males (blue), dilp5-Gal4/+;UAS-DifRNAi/+ males (brown), wor-Gal4/+;UAS-DifRNAi/+ males (orange), dilp5-Gal4,wor-Gal4/+;UAS-DifRNAi/+ males (magenta), and +/+;UAS-DifRNAi/+ (black) control males. Corresponding lifespan analysis (G) and bodyweight (H) for females. Data information: statistics for curve comparisons are shown in the figure. Error bars represent mean ± SEM. *** p<.001, **** p<.0001 (log-rank test). n = at least 100 flies for each genotype in lifespan experiments. n = 4 vials of 15 flies for each genotype for bodyweight measurements.