Research Paper Volume 13, Issue 1 pp 424—436

LncRNA HAND2-AS1 suppressed the growth of triple negative breast cancer via reducing secretion of MSCs derived exosomal miR-106a-5p

Exo-miR-106a-5p derived from MSCs promoted migration, invasion and proliferation of TNBC cells. BT549 and MDA-MB-231 cells were incubated with exosomes from MSCs transfected miR-106a-5p or AMO-miR-106a-5p or its NC. (A, B) MTT was used to test viability of BT549 and MDA-MB-231 cells. n = 10, *pC, D) Wound healing assay to detect migration ability. n = 4, *pE, F) Transwell assay to detect invasion ability. n = 4, *pG, H) Clone formation assay to detect proliferation ability. n = 4, *p

Figure 3. Exo-miR-106a-5p derived from MSCs promoted migration, invasion and proliferation of TNBC cells. BT549 and MDA-MB-231 cells were incubated with exosomes from MSCs transfected miR-106a-5p or AMO-miR-106a-5p or its NC. (A, B) MTT was used to test viability of BT549 and MDA-MB-231 cells. n = 10, *p<0.05 vs MSC-miR-NC or MSC-AMO-miR-NC. (C, D) Wound healing assay to detect migration ability. n = 4, *p<0.05 vs MSC-miR-NC or MSC-AMO-miR-NC. (E, F) Transwell assay to detect invasion ability. n = 4, *p<0.05 vs MSC-miR-NC or MSC-AMO-miR-NC. (G, H) Clone formation assay to detect proliferation ability. n = 4, *p<0.05 vs MSC-miR-NC or MSC-AMO-miR-NC. The above measurement data were expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA, followed by a Tukey post hoc test. The experiment was repeated in triplicate.