Research Paper Volume 13, Issue 1 pp 424—436

LncRNA HAND2-AS1 suppressed the growth of triple negative breast cancer via reducing secretion of MSCs derived exosomal miR-106a-5p

HAND2-AS1 inhibited TNBC tumorigenesis in vivo. BT549 and MDA-MB-231 cells were incubated with exosomes from MSCs transfected with miR-106a-5p, then were injected into nude mice. 1 week later, lentivirus packaging HAND2-AS1 was injected into tumors. (A,.B) Growth of tumor xenografts in nude mice. n = 6, *pC) Representative tumors excised from xenografts in nude mice and tumor weight, and the ratio of tumor weight to body weight was calculated. n = 6, *pD) Expression of Ki67 by immunohistochemical staining in tumors. n = 6, *pE, F) The expression of miR-106a-5p and HAND2-AS1 in tumors were detected by qRT-PCR. n = 6, *p

Figure 7. HAND2-AS1 inhibited TNBC tumorigenesis in vivo. BT549 and MDA-MB-231 cells were incubated with exosomes from MSCs transfected with miR-106a-5p, then were injected into nude mice. 1 week later, lentivirus packaging HAND2-AS1 was injected into tumors. (A,.B) Growth of tumor xenografts in nude mice. n = 6, *p<0.05. (C) Representative tumors excised from xenografts in nude mice and tumor weight, and the ratio of tumor weight to body weight was calculated. n = 6, *p<0.05. (D) Expression of Ki67 by immunohistochemical staining in tumors. n = 6, *p<0.05. (E, F) The expression of miR-106a-5p and HAND2-AS1 in tumors were detected by qRT-PCR. n = 6, *p<0.05. The above measurement data were expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA, followed by a Tukey post hoc test. The experiment was repeated in triplicate.