Research Paper Volume 13, Issue 1 pp 493—515

Melatonin alleviates angiotensin-II-induced cardiac hypertrophy via activating MICU1 pathway

MICU1 overexpression in the heart attenuated cardiac hypertrophy. (A, B) Representative immunoblots and quantification of protein levels of MICU1 in control (A) and hypertrophy hearts (B) infected with Ad-EV and Ad-MICU1 were shown. (C) Histological analyses of heart sections stained with H&E, wheat germ agglutinin, and Masson from the indicated groups were presented. (D) The average cross-sectional area of cardiomyocytes from the indicated groups was summarized. (E) The interstitial fibrosis was quantified. (F, G) The rations of HW/TL (F) and LVW/TL (G) in different mice were determined. (H) Representative echocardiographic image of the left ventricle in different mice was represented. (I–K) Echocardiographic assessment of LVEDd, LVESd and FS was used to reflect cardiac function. Ad-EV, control adenovirus; Ad-MICU1, recombinant adenovirus encoding MICU1. All the data represent the means ± SEM. N=6-8/group. **P##P&P&&PΨPΨΨP

Figure 4. MICU1 overexpression in the heart attenuated cardiac hypertrophy. (A, B) Representative immunoblots and quantification of protein levels of MICU1 in control (A) and hypertrophy hearts (B) infected with Ad-EV and Ad-MICU1 were shown. (C) Histological analyses of heart sections stained with H&E, wheat germ agglutinin, and Masson from the indicated groups were presented. (D) The average cross-sectional area of cardiomyocytes from the indicated groups was summarized. (E) The interstitial fibrosis was quantified. (F, G) The rations of HW/TL (F) and LVW/TL (G) in different mice were determined. (H) Representative echocardiographic image of the left ventricle in different mice was represented. (IK) Echocardiographic assessment of LVEDd, LVESd and FS was used to reflect cardiac function. Ad-EV, control adenovirus; Ad-MICU1, recombinant adenovirus encoding MICU1. All the data represent the means ± SEM. N=6-8/group. **P<0.01 vs. Vehicle in Control; ##P<0.01 vs. Vehicle in Hypertrophy; &P<0.05, &&P<0.01 vs. Control; ΨP<0.05, ΨΨP<0.01 vs. Ad-EV of hypertrophy.