Research Paper Volume 12, Issue 24 pp 25939—25955

Sexual dimorphism in aging hematopoiesis: an earlier decline of hematopoietic stem and progenitor cells in male than female mice

Figure 4. Sex-mismatched heterochronic BM transplantations. (A) Schematic of the experimental design of sex-mismatched heterochronic BM transplantations. Total BM cells were isolated from male and female CD45.1 young donor mice, and transplanted into lethally irradiated middle-aged female or male C57BL/6J recipient mice. After 16 weeks, CD45.1+ donor cell chimerism was determined and donor-derived lineage-Sca-1+c-kit+ (LSK) cells were isolated per flow cytometry cell sorting. (B) Yng-M donor cell chimerisms in Mid-F and Mid-M were comparable and ≥ 90%. (C) Yng-F donor cell chimerisms in Mid-F and Mid-M were also comparable and ≥ 90%. (D) A considerable number of hematopoietic genes (represented by yellow dots) in the Yng-M-derived LSK cells from the Mid-F recipients exhibited significantly higher expression than from the Mid-M recipients. (E) A number of hematopoietic genes (represented by yellow dots) in the Yng-F-derived LSK cells from the Mid-F recipients exhibited significantly higher expression than from the Mid-M recipients. Yellow dots indicate genes with potentially higher level of expression in mice of y-axis than that in mice of x-axis; black dots indicate genes with comparable level of expression; blue dots indicate genes with potentially lower level of expression in mice of y-axis than that in mice of x-axis.