Figure 3. Knockdown of MITF increases metastasis and tumorigenesis but inhibits angiogenesis. (A) Intravenous injection with stable shMITF-harboring and scramble CL1-0 cells to SCID mice. The lung metastases were estimated by using real-time PCR for detection of the human Alu repeats. (B) Subcutaneous injection with stable shMITF-harboring cells into the right dorsal region and scramble cells into the left dorsal region of SCID mice. The tumor volume was measured every week. The mouse number of each group is listed on the plot. *p < 0.05 was calculated by ANOVA (mean ± SD) (C) The cells mixed with Matrigel and injected subcutaneously. At 10 days, the plug was dissected and assayed the CD31 positive cells by immunochemistry. Scale Bar, 25 μm. (D) The CD31 positive cells were counted by two blinded observers. A total of 21 fields/group (3 plugs × 7 fields) were analyzed. *p<0.05 (mean ± SD).