Research Paper Volume 13, Issue 1 pp 694—713

Activation of adenosine A3 receptor reduces early brain injury by alleviating neuroinflammation after subarachnoid hemorrhage in elderly rats

Activation of A3R induced the expression of Arg-1 in vitro, and enhanced the activation of P38, STAT6 both in vitro and in vivo. (A) Western blot analysis showing the expression of Arg-1 and phosphorylated JAK1, STAT1, P38, STAT6 and NF-κB P65 24 h after OxyHb treatment. The protein levels were quantified. (B) Effects of the A3R agonist (CI-IB-MECA) and antagonist (MRS1523) on the P38/STAT6 pathway, IL-13 and IL-4(STAT6 activator) in vivo 24 h after SAH. Western blot analysis showing the protein of phosphorylated P38 (p-P38), STAT6 (p-STAT6), IL-13 and IL-4 under the indicated treatments. (C) Effect of CI-IB-MECA on the expression of IL-13 and IL-4, in neuron or microglia in vitro. β-Tubulin was used as a loading control. n=5 in each group. Data are shown as the mean ± SD. **p p p p

Figure 5. Activation of A3R induced the expression of Arg-1 in vitro, and enhanced the activation of P38, STAT6 both in vitro and in vivo. (A) Western blot analysis showing the expression of Arg-1 and phosphorylated JAK1, STAT1, P38, STAT6 and NF-κB P65 24 h after OxyHb treatment. The protein levels were quantified. (B) Effects of the A3R agonist (CI-IB-MECA) and antagonist (MRS1523) on the P38/STAT6 pathway, IL-13 and IL-4(STAT6 activator) in vivo 24 h after SAH. Western blot analysis showing the protein of phosphorylated P38 (p-P38), STAT6 (p-STAT6), IL-13 and IL-4 under the indicated treatments. (C) Effect of CI-IB-MECA on the expression of IL-13 and IL-4, in neuron or microglia in vitro. β-Tubulin was used as a loading control. n=5 in each group. Data are shown as the mean ± SD. **p < 0.01, versus sham group; ## p < 0.01, versus OxyHb group or SAH group; & p < 0.05 and && p < 0.01 versus OxyHb +CI-IB-MECA group or SAH+CI-IB-MECA group.