Research Paper Volume 13, Issue 1 pp 769—781

The microRNA miR-29c-5p inhibits cell proliferation and migration by targeting TMEM98 in head and neck carcinoma

miR-29c-5p regulates the proliferation of FaDu and HSC-3 cells. (A) The levels of miR-29c-5p were significantly higher in FaDu and HSC-3 cells transfected with the miR-29c-5p mimic but lower in cells transfected with the miR-29c-5p inhibitor. (B, C) The results of CCK-8 assays suggest that upregulated miR-29c-5p expression induced by its cognate mimic significantly decreased the cell growth rate compared with the control. Conversely, the growth rates of FaDu and HSC-3 cells transfected with the miR-29c-5p inhibitor were significantly higher compared with the negative control. (D–F) Cell cycle analysis of FaDu and HSC-3 cells transfected with the miR-29c-5p inhibitor shows that the percentages of S-phase cells significantly increased while the percentages of G2/M-phase cells decreased compared with the control (p

Figure 2. miR-29c-5p regulates the proliferation of FaDu and HSC-3 cells. (A) The levels of miR-29c-5p were significantly higher in FaDu and HSC-3 cells transfected with the miR-29c-5p mimic but lower in cells transfected with the miR-29c-5p inhibitor. (B, C) The results of CCK-8 assays suggest that upregulated miR-29c-5p expression induced by its cognate mimic significantly decreased the cell growth rate compared with the control. Conversely, the growth rates of FaDu and HSC-3 cells transfected with the miR-29c-5p inhibitor were significantly higher compared with the negative control. (DF) Cell cycle analysis of FaDu and HSC-3 cells transfected with the miR-29c-5p inhibitor shows that the percentages of S-phase cells significantly increased while the percentages of G2/M-phase cells decreased compared with the control (p<0.05). Conversely, upregulation of miR-29c-5p expression decreased and increased the percentages of cells in S phase and G2/M phase, respectively. *p < 0.05, **p < 0.01, ***p < 0.001.