Figure 4. miR-29c-5p directly targets the 3´-UTR of TMEM98 mRNA to modulate TMEM98 expression. (A) Analysis of the three bioinformatics databases showing the number of potential has-miR-29c-target genes. (B) Relative mRNA levels of five potential has-miR-29c-target genes in cells transfected with the has-miR-29c mimic vs the control. (C) The Upper is the putative binding site of miR-29c-5p in the 3´-UTR of TMEM98 mRNA. The lower is the relative luciferase activities of wild-type and mutant TMEM98 reporters in FaDu and HSC-3 cells. (D, E) mRNA and protein levels of TMEM98 in FaDu and HSC-3 cells transfected with the miR-29c-5p mimic or inhibitor. (F, G) mRNA levels of TMEM98 and their association with has-miR-29c levels in HNSCC tissues. *p < 0.05, **p < 0.01.