Research Paper Volume 13, Issue 1 pp 865—876

Propofol reduces renal ischemia/reperfusion-induced acute lung injury by stimulating sirtuin 1 and inhibiting pyroptosis

Propofol reduces the rI/R-induced release of pyroptotic proteins and inflammatory cytokines in rat alveolar macrophages. NR8383 cells were incubated with serum from sham or rI/R rats, with or without propofol (50 or 100 μM) for 24 h. (A, B) Western blotting was used to determine the levels of cleaved caspase 1 (P10), ASC and NLRP3. (C, D) ELISAs were used to determine the protein levels of IL-1β and IL-18 (*P #P

Figure 1. Propofol reduces the rI/R-induced release of pyroptotic proteins and inflammatory cytokines in rat alveolar macrophages. NR8383 cells were incubated with serum from sham or rI/R rats, with or without propofol (50 or 100 μM) for 24 h. (A, B) Western blotting was used to determine the levels of cleaved caspase 1 (P10), ASC and NLRP3. (C, D) ELISAs were used to determine the protein levels of IL-1β and IL-18 (*P < 0.05 vs. sham; #P < 0.05 vs. rI/R).