Figure 4. Coicis semen alleviated blood-brain barrier (BBB) leakage after ischemic stroke. (A) Images showing Evans blue (EB) dye leakage in brains after EB injection (by tail vein). (B) EB dye extravasation 2 h after tail vein injection of EB dye was quantitatively analyzed using a spectrophotometer. (C) Western blot analysis showing that Coicis Semen treatment increased the expression of ZO-1 and Occludin. (D) Quantitative analysis of the ZO-1 protein levels. (E) Quantitative analysis of the Occludin protein levels. Mean ± SD. n = 5. aP < 0.05 vs. vehicle. Statistical analyses were performed by two-tailed Student’s t-test.