Figure 3. MiR-1272 directly targets ADAM9 and regulates its expression in vitro. (A) The binding sites of miR-1272 and ADAM9. (B) Luciferase activities decreased upon transfection with miR-1272 and ADAM9 3'-UTR luciferase reporter vector in HEK-293 and U251 cells. (C) MiR-1272 regulated the protein expression of ADAM9 detected by western blotting. Null, psiCHECKTM-2 report plasmid without ADAM9 3'-UTR. Error bars represent mean ± SEM. *** p < 0.001.