Research Paper Volume 13, Issue 1 pp 910—932

Tanshinone IIA attenuates atherosclerosis via inhibiting NLRP3 inflammasome activation

Effects of the LOX-1, CD36 and SR-A1 siRNAs on oxLDL-induced IL-1β productions and effects of oxLDL and Tan IIA on LOX-1 expression and LOX-1-mediated caspase-1activation/IL-1β release. (A–C) Effects of lentivirus-mediated LOX-1, CD36 and SR-A1 siRNAs (MOI = 30) on oxLDL-induced IL-1β productions in mouse macrophages measured by ELISA. (D, E) Effects of oxLDL on LOX-1 expression in macrophages measured by qRT-PCR and Western blot, respectively. (F, G) Effects of Tan IIA on oxLDL-induced LOX-1 expression determined by qRT-PCR and Western blot, respectively. (H) Effects of Tan IIA on oxLDL-induced LOX-1-mediated IL-1β expression in macrophages. (I) Effects of Tan IIA on oxLDL-induced LOX-1-mediated caspase-1 activation in macrophages.

Figure 3. Effects of the LOX-1, CD36 and SR-A1 siRNAs on oxLDL-induced IL-1β productions and effects of oxLDL and Tan IIA on LOX-1 expression and LOX-1-mediated caspase-1activation/IL-1β release. (AC) Effects of lentivirus-mediated LOX-1, CD36 and SR-A1 siRNAs (MOI = 30) on oxLDL-induced IL-1β productions in mouse macrophages measured by ELISA. (D, E) Effects of oxLDL on LOX-1 expression in macrophages measured by qRT-PCR and Western blot, respectively. (F, G) Effects of Tan IIA on oxLDL-induced LOX-1 expression determined by qRT-PCR and Western blot, respectively. (H) Effects of Tan IIA on oxLDL-induced LOX-1-mediated IL-1β expression in macrophages. (I) Effects of Tan IIA on oxLDL-induced LOX-1-mediated caspase-1 activation in macrophages.