Research Paper Volume 13, Issue 1 pp 910—932

Tanshinone IIA attenuates atherosclerosis via inhibiting NLRP3 inflammasome activation

Tan IIA inhibits oxLDL-induced LOX-1-mediated ROS production and mitochondrial damage in macrophages. (A) Oxidized LDL induction and Tan IIA inhibition on ROS generation detected using ROS fluorescence detector DCFH-DA (ATP treatment as a positive control). (B, C) Oxidized LDL induction and Tan IIA inhibition on mito-ROS production detected with MitoSOX (Invitrogen) using a flow cytometer in mouse B6 macrophages and LOX-1-KD B6 macrophages, respectively. (D) Mito-ROS production in the B6 macrophages induced by oxLDL at the different doses (0, 10, 25, 50, 100 μg/mL).

Figure 4. Tan IIA inhibits oxLDL-induced LOX-1-mediated ROS production and mitochondrial damage in macrophages. (A) Oxidized LDL induction and Tan IIA inhibition on ROS generation detected using ROS fluorescence detector DCFH-DA (ATP treatment as a positive control). (B, C) Oxidized LDL induction and Tan IIA inhibition on mito-ROS production detected with MitoSOX (Invitrogen) using a flow cytometer in mouse B6 macrophages and LOX-1-KD B6 macrophages, respectively. (D) Mito-ROS production in the B6 macrophages induced by oxLDL at the different doses (0, 10, 25, 50, 100 μg/mL).