Figure 2. PVT1 acted as the oncogene. The relative expression level of PVT1 was reduced by si-PVT1 (A) and increased by pcDNA3.1-PVT1 (B). The relative expression level of miR-194-5p was reduced by pcDNA3.1-PVT1 (C). Cell proliferation was detected in both BC cells after transfection of siRNA (D, E) and pcDNA3.1-PVT1 (F). Representative images of EdU assay and the relative fold changes of EdU positive cells were detected by siRNA (G, H) and pcDNA3.1-PVT1 (I). (*P < 0.05, **P < 0.01).