Research Paper Volume 13, Issue 1 pp 933—943

IRF-1 contributes to the pathological phenotype of VSMCs during atherogenesis by increasing CCL19 transcription

Effects of CCL19 on VSMCS inflammation, ECM deposition, proliferation and migration. (A) Detection of overexpression efficiency of CCL19 plasmid was measured by qRT-PCR. The inflammatory cytokine IL1-α (B), IL-1β (C), TNF-α (D), IL-6 (E) and the deposition of ECM such as collagen1 (F), collagen3 (G) and OPN (H) were detected by qRT-PCR.n=5 (I) Western blot showed the expression of proteins collagen1 and IL-6. n=4. Migration (J) and proliferation (K) of VSMCS after overexpressing CCL19. n=6. EV: Empty vector. *P

Figure 2. Effects of CCL19 on VSMCS inflammation, ECM deposition, proliferation and migration. (A) Detection of overexpression efficiency of CCL19 plasmid was measured by qRT-PCR. The inflammatory cytokine IL1-α (B), IL-1β (C), TNF-α (D), IL-6 (E) and the deposition of ECM such as collagen1 (F), collagen3 (G) and OPN (H) were detected by qRT-PCR.n=5 (I) Western blot showed the expression of proteins collagen1 and IL-6. n=4. Migration (J) and proliferation (K) of VSMCS after overexpressing CCL19. n=6. EV: Empty vector. *P<0.05; **P<0.01.