Research Paper Volume 13, Issue 1 pp 957—972

Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells

The activation of hepatic stellate cells induced Bnip3 expression and its cytoplasmic translocation in vitro. LX-2 cells were treated with 100 μM CoCl2 or 2 μg/ml LPS for 8 h. (A) Cells were collected at indicated time and cell lysates were subjected to detect Bnip3 with Western blot. Densitometric analysis for Western blot was performed and data were expressed as mean ± SD, *P B) Immunofluorescence assay was performed to detect Bnip3 (Cy3) in CoCl2- or LPS-treated LX-2 cells. Culture-activated primary HSCs from mice were cultured up to 2 days, 4 days or 6 days. (C) Cells were collected at indicated time and cell lysates were subjected to detect Bnip3 with Western blot. Densitometric analysis for Western blot was performed and data were expressed as mean ± SD, *P P D) Immunofluorescence assay was performed to detect Bnip3 (Cy3) and images were captured by confocal microscope.

Figure 2. The activation of hepatic stellate cells induced Bnip3 expression and its cytoplasmic translocation in vitro. LX-2 cells were treated with 100 μM CoCl2 or 2 μg/ml LPS for 8 h. (A) Cells were collected at indicated time and cell lysates were subjected to detect Bnip3 with Western blot. Densitometric analysis for Western blot was performed and data were expressed as mean ± SD, *P < 0.05. (B) Immunofluorescence assay was performed to detect Bnip3 (Cy3) in CoCl2- or LPS-treated LX-2 cells. Culture-activated primary HSCs from mice were cultured up to 2 days, 4 days or 6 days. (C) Cells were collected at indicated time and cell lysates were subjected to detect Bnip3 with Western blot. Densitometric analysis for Western blot was performed and data were expressed as mean ± SD, *P < 0.05, **P < 0.01. (D) Immunofluorescence assay was performed to detect Bnip3 (Cy3) and images were captured by confocal microscope.