Figure 3. Autophagy occurred when hepatic stellate cells were activated. (A) LX-2 cells were treated with 100 μM CoCl2 or 2 μg/ml LPS for 8 h. (B) Culture-activated primary HSCs from mice were cultured up to 2 days or 6 days. Immunofluorescence assay was performed to detect LC3B (FITC) in CoCl2- or LPS-treated LX-2 cells (A) and culture-activated primary HSCs from mice cultured up to 2 days or 6 days (B). Images were captured by confocal microscope. (C) Culture-activated primary HSCs from mice were cultured up to 2 days, 4 days or 6 days. Cells were collected at indicated time and cell lysates were subjected to detect Hif-1α, α-SMA and LC3B with Western blot. Densitometric analysis for Western blot was performed and data were expressed as mean ± SD, *P < 0.05, ***P < 0.001.