Research Paper Volume 12, Issue 24 pp 26000—26011

Suppression of long noncoding RNA LINC00324 restricts cell proliferation and invasion of papillary thyroid carcinoma through downregulation of TRIM29 via upregulating microRNA-195-5p

Figure 6. TRIM29 overexpression reverses the miR-195-5p-overexpression- or LINC00324-knockdown-mediated effects in PTC cells. miR-195-5p mimic and pcDNA3.1/TRIM29 vectors were cotransfected into TPC1 cells, and (A) protein levels of TRIM29 were examined via western blot. (B) Effect of TRIM29 overexpression on miR-195-5p-mediated cellular proliferation was determined via a CCK-8 assay. (C, D) Effect of TRIM29 overexpression on miR-195-5p-mediated cellular invasion was evaluated via a transwell invasion assay. LINC00324 siRNA and pcDNA3.1/TRIM29 vectors were cotransfected into TPC1 cells, and (E) protein levels of TRIM29 was examined via western blot. (F) Effect of TRIM29 overexpression on LINC00324 knockdown-mediated cellular proliferation was measured via a CCK-8 assay. (G, H) Effect of TRIM29 overexpression on LINC00324 knockdown-mediated cellular invasion was monitored via a transwell invasion assay. *p < 0.05.