Research Paper Volume 13, Issue 1 pp 1017—1031

AIM2 inhibits colorectal cancer cell proliferation and migration through suppression of Gli1

AIM2 protein expression in human CRC cell lines. (A) Western blots of AIM2 protein in five human CRC cell lines (HCT116, CCL244, SW480, SW620 and LoVo). GAPDH as a loading control. (B) Western blots of AIM2 protein in SW480 and SW620 cells stably transfected with control-shRNA (NC) or shRNA against AIM2 (KD). GAPDH as a loading control. Each experiment was performed at least triplicate and the bands were quantified and presented as the mean±SEM. (C) Western blots of AIM2 protein in HCT116 and LoVo cells stably transfected with empty vector (VEC) or plasmid encoding human AIM2 (AIM2). GAPDH as a loading control. Each experiment was performed at least triplicate and the bands were quantified and presented as the mean±SEM. **P

Figure 2. AIM2 protein expression in human CRC cell lines. (A) Western blots of AIM2 protein in five human CRC cell lines (HCT116, CCL244, SW480, SW620 and LoVo). GAPDH as a loading control. (B) Western blots of AIM2 protein in SW480 and SW620 cells stably transfected with control-shRNA (NC) or shRNA against AIM2 (KD). GAPDH as a loading control. Each experiment was performed at least triplicate and the bands were quantified and presented as the mean±SEM. (C) Western blots of AIM2 protein in HCT116 and LoVo cells stably transfected with empty vector (VEC) or plasmid encoding human AIM2 (AIM2). GAPDH as a loading control. Each experiment was performed at least triplicate and the bands were quantified and presented as the mean±SEM. **P<0.01, based on a two-tailed paired Student’s t-test.